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Addgene

pBK2
(Plasmid #1214)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1214 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Bluescribe
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3200
  • Vector type
    Yeast Expression ; integration

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Unknown
  • Growth instructions
    BSJ72
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Hsp82::LEU2
  • Alt name
    hsp90
  • Alt name
    heat shock
  • Alt name
    hsp82
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2800
  • Mutation
    hsp82::LEU2 select for on LEU2 excise fragment using HindIII and MluI
  • GenBank ID
    NC_001148
  • Entrez Gene
    HSP82 (a.k.a. YPL240C, HSP90)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBK2 was a gift from Susan Lindquist (Addgene plasmid # 1214 ; http://n2t.net/addgene:1214 ; RRID:Addgene_1214)

  • For your References section:

    Mutational analysis of Hsp90 function: interactions with a steroid receptor and a protein kinase. Nathan DF, Lindquist S. Mol Cell Biol 1995 Jul;15(7):3917-25. PubMed 7791797
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