pHMT.wt (p53)
(Plasmid #12139)

• Depositing Lab: James Sherley
• Publication: Liu et al J Cell Physiol. 1998 Nov . 177(2):364-76.

Backbone

• Vector backbone: pM2.6
• Backbone manufacturer: M.J. Sleigh (CSIRO)
• Backbone size w/o insert (bp): 4630
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p53
• Species: M. musculus (mouse)
• Insert Size (bp): 1500
• Entrez Gene: Trp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: na

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Murine wild-type p53 cDNA was excised from plasmid
pLCRcala (Sherley, 1991) by digestion with BamHI and TfiI. The restriction fragment ends were filled in with Klenow and ligated to HindIII linkers.
Following digestion with HindIII, the p53 fragment was inserted into the HindIII site of the
plasmid pM2.6, downstream of the Zn-responsive
human metallothionein promoter modified to provide
low levels of basal gene expression (McNeall et al.,
1989).