pLCR0
(Plasmid #12137)

• Purpose: (Empty Backbone)
• Depositing Lab: James Sherley
• Publication: Sherley et al J Biol Chem. 1991 Dec 25. 266(36):24815-28.

Backbone

• Vector backbone: na
• Backbone size (bp): 5010
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: SV40pro-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Arnold J. Levine (Princeton)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid, containing
the previously
described BPV LCR-SV40 hybrid promoter (Spaholz et al., 1985),
was constructed as follows. Plasmid p407-1 (Spaholz et al., 1985) was
digested with EcoRI and HindIII, and the 5010-base pair fragment
containing the LCR-SV40 hybrid promoter was isolated and filled in with Klenow. BamHI linkers were ligated to the ends of the fragment, and the fragment was circularized.