pcDNA3.1 myc TSC1
(Plasmid #12133)

• Depositing Lab: Cheryl Walker
• Publication: Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

Backbone

• Vector backbone: pcDNA3.1A Myc-His
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TSC1
• Species: H. sapiens (human)
• Insert Size (bp): 3500
• Entrez Gene: TSC1 (a.k.a. LAM, TSC)
• Tags / Fusion Proteins:
  • myc (C terminal on backbone)
  • his (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Jeff DeClue
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results found a deletion of S1043 when compared to TSC1 reference sequences such as GenBank NP_001155898.1 (isoform 3) and NP_001155899.1 (isoform 4).