Flag TSC2 wt
(Plasmid #12132)

• Depositing Lab: Cheryl Walker
• Publication: Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

Backbone

• Vector backbone: pCMV-Tag2
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4300
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TSC2
• Species: H. sapiens (human)
• Insert Size (bp): 5400
• GenBank ID:
• Entrez Gene: TSC2 (a.k.a. LAM, PPP1R160, TSC4)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ShaoWei Li (Dr. Jeff DeClue's lab)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Compared to GenBank reference sequence NP_001107854.1, the TSC2 insert contains a S802R mutation.