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Flag TSC2 2A(S939A + S981A)
(Plasmid #12131)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12131 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Tag2
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TSC2 S939A S981A
  • Alt name
    TSC2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5400
  • Mutation
    S939A. S981A.
  • GenBank ID
    X75621
  • Entrez Gene
    TSC2 (a.k.a. LAM, PPP1R160, TSC4)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ShaoWei Li (Dr. Jeff DeClue's lab)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag TSC2 2A(S939A + S981A) was a gift from Cheryl Walker (Addgene plasmid # 12131 ; http://n2t.net/addgene:12131 ; RRID:Addgene_12131)

  • For your References section:

    Activity of TSC2 is inhibited by AKT-mediated phosphorylation and membrane partitioning. Cai SL, Tee AR, Short JD, Bergeron JM, Kim J, Shen J, Guo R, Johnson CL, Kiguchi K, Walker CL. J Cell Biol. 2006 Apr 24. 173(2):279-89. 10.1083/jcb.200507119 PubMed 16636147
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