pAcGP67A-TfR
(Plasmid #12130)

• Depositing Lab: Pamela Bjorkman
• Publication: Lebron et al Cell. 1998 Apr 3. 93(1):111-23.

Backbone

• Vector backbone: pAcGP67A
• Backbone manufacturer: Pharmingen
• Backbone size w/o insert (bp): 9761
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: transferrin receptor
• Alt name: TfR
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Mutation: The portion of the human TfR gene encoding residues 121-760 were fused 3' to a gene segment encoding the hydrophobic leader peptide from the baculovirus protein gp67, a 6X-His tag, and a factor Xa cleavage site in a modified version of the pAcG67A expression vector.
• GenBank ID: NP_003225
• Entrez Gene: TFRC (a.k.a. CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90)
• Tags / Fusion Proteins:
  • leader peptide from baculovirus protein gp67 (N terminal on insert)
  • 6X-His tag (N terminal on insert)
  • Factor Xa cleavage site (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: 5' AAT TTAA AAT gAT AAC CAT CTC gC 3'
• 3′ sequencing primer: 5' gAA gCg ggT CCA AgT TTC CCT g 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry