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pUC57-Kan_BR29_00096171
(Plasmid #121214)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121214 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57-Kan
  • Backbone size w/o insert (bp) 2626
  • Vector type
    Golden Gate
  • Selectable markers
    lacZ

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BR29_00096171
  • Mutation
    Magnaporthe grisea

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (unknown if destroyed)
  • 3′ cloning site EcoRV (unknown if destroyed)
  • 5′ sequencing primer M13Fw
  • 3′ sequencing primer M13Rv
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57-Kan_BR29_00096171 was a gift from Sophien Kamoun (Addgene plasmid # 121214 ; http://n2t.net/addgene:121214 ; RRID:Addgene_121214)

  • For your References section:

    A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence and Structural Similarities Across Host-Specific Lineages. Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S. Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22. 10.1094/MPMI-03-20-0052-A PubMed 32460610
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