PEX-LV
(Plasmid #12120)

• Depositing Lab: Inder Verma
• Publication: Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 97(22):12227-32.

Backbone

• Vector backbone: p156RRLsinPPT
• Backbone manufacturer: Salk/Cell Genesys
• Backbone size w/o insert (bp): 7424
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Matrix Metalloproteinase 2
• Alt name: MMP2
• Alt name: PEX
• Species: M. musculus (mouse)
• Insert Size (bp): 663
• Mutation: noncatalytic fragment of MMP2 comprised of the first 29aa N-terminal leader sequence and aa441-633 of the hemopexin domain
• Entrez Gene: Mmp2 (a.k.a. Clg4a, GelA, MMP-2)
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry