pCUP1-GR
(Plasmid #1212)

• Depositing Lab: Susan Lindquist
• Publication: Li et al Science 2000 Jan 28;287(5453):661-4.

Backbone

• Vector backbone: pRS316
• Backbone size w/o insert (bp): 4848
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: glucocorticoid receptor
• Alt name: GR
• Species: R. norvegicus (rat)
• Insert Size (bp): 2840
• Entrez Gene: Nr3c1 (a.k.a. GR, Gcr, Grl)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NM domain removed from pCUP1-NMGR with BamHI/SacII digest (not destroyed)
• 3′ cloning site: NM domain removed from pCUP1-NMGR with BamHI/SacII digest (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: see comments.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Derived from plasmids from the following reference: Picard D, Khurseed B, Garabedian MJ, Fortin MG, Lindquist S, Yamamoto
KR. Reduced levels of hsp90 compromise steroid receptor action in
vivo. Nature (1990), 348 (6297): 166-8.

Note: Please see Addgene's sequencing result for the GR sequence, which is slightly different from the author's sequence.