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Addgene

pMK365 (Tet-OsTIR1-loxP-PURO-loxP)
(Plasmid #121185)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121185 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tet-OsTIR1-loxP-PURO-loxP
  • Species
    Synthetic
  • Mutation
    OsTIR1 codon optimized for human cells

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is a variant of Addgene plasmid 72835.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMK365 (Tet-OsTIR1-loxP-PURO-loxP) was a gift from Masato Kanemaki (Addgene plasmid # 121185 ; http://n2t.net/addgene:121185 ; RRID:Addgene_121185)

  • For your References section:

    Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Yesbolatova A, Natsume T, Hayashi KI, Kanemaki MT. Methods. 2019 Apr 23. pii: S1046-2023(18)30331-1. doi: 10.1016/j.ymeth.2019.04.010. 10.1016/j.ymeth.2019.04.010 PubMed 31026591
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