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PurposeOsTIR1 introduction
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 121184 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCMV-OsTIR1-loxP-PURO-loxP
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SpeciesSynthetic
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MutationOsTIR1 codon optimized for human cells
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is a variant of Addgene plasmid 72834.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMK364 (CMV-OsTIR1-loxP-PURO-loxP) was a gift from Masato Kanemaki (Addgene plasmid # 121184 ; http://n2t.net/addgene:121184 ; RRID:Addgene_121184) -
For your References section:
Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Yesbolatova A, Natsume T, Hayashi KI, Kanemaki MT. Methods. 2019 Apr 23. pii: S1046-2023(18)30331-1. doi: 10.1016/j.ymeth.2019.04.010. 10.1016/j.ymeth.2019.04.010 PubMed 31026591
