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lentiCRISPRv2_PROX1_1+4
(Plasmid #121177)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121177 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentiCRISPR v2 (Plasmid #52961)
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
  • Species
    Streptococcus pyogenes
  • Tag / Fusion Protein
    • P2A-puro

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Modified version of lentiCRISPR v2 (Plasmid #52961) to incorporate the expression of two gRNAs from two independent U6 promoters.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

gRNA 1: GCTCAAGAATCCCGGGACCC
gRNA 4: CGGGTTGAGAATATAATTCG

Used to KO PROX1 in Högström et al. 2018 (doi: 10.1158/0008-5472.CAN-18-0451.)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lentiCRISPRv2_PROX1_1+4 was a gift from Timo Otonkoski (Addgene plasmid # 121177 ; http://n2t.net/addgene:121177 ; RRID:Addgene_121177)
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