pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro
(Plasmid
#121175)
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PurposeDonor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 121175 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 2647
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Vector typeMammalian Expression, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
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SpeciesH. sapiens (human)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro was a gift from Timo Otonkoski (Addgene plasmid # 121175 ; http://n2t.net/addgene:121175 ; RRID:Addgene_121175)
