CAG-ABEmax-P2A-EGFP-ires-puro
(Plasmid
#121170)
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PurposeCAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 121170 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePyCAG
- Total vector size (bp) 12644
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Vector typeMammalian Expression, CRISPR
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Selectable markersPuromycin ; EGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameABEmax-P2A-EGFP-ires-puro
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SpeciesStreptococcus pyogenes
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Insert Size (bp)6195
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Tag
/ Fusion Protein
- P2A-EGFP-ires-puro (C terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byThe ABEmax-P2A fragment was cloned from pCMV_ABEmax_P2A_GFP (Plasmid #112101) from David Liu lab.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CAG-ABEmax-P2A-EGFP-ires-puro was a gift from Timo Otonkoski (Addgene plasmid # 121170 ; http://n2t.net/addgene:121170 ; RRID:Addgene_121170)
