CAG-FNLS-T2A-EGFP-ires-puro
(Plasmid
#121169)
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PurposeCAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 121169 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePyCAG
- Total vector size (bp) 12644
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Vector typeMammalian Expression, CRISPR
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Selectable markersPuromycin ; EGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFNLS-T2A-EGFP-ires-puro
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SpeciesStreptococcus pyogenes
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Insert Size (bp)6042
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Tag
/ Fusion Protein
- T2A-EGFP-ires-puro (C terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byBE3 FNLS fragment cloned from pLenti-FNLS-P2A-GFP-PGK-Puro (Plasmid #110869) from Lukas Dow lab.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CAG-FNLS-T2A-EGFP-ires-puro was a gift from Timo Otonkoski (Addgene plasmid # 121169 ; http://n2t.net/addgene:121169 ; RRID:Addgene_121169)
