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PurposepMP71 vector expressing GFP - P2A - G5 - myc - CD40
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 121166 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMP71
- Backbone size w/o insert (bp) 6250
- Total vector size (bp) 7250
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameG5-CD40
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SpeciesM. musculus (mouse)
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Insert Size (bp)1689
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Tags
/ Fusion Proteins
- myc (N terminal on insert)
- GFP (N terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
G5-CD40 was a gift from Gabriel Victora (Addgene plasmid # 121166 ; http://n2t.net/addgene:121166 ; RRID:Addgene_121166) -
For your References section:
Monitoring T cell-dendritic cell interactions in vivo by intercellular enzymatic labelling. Pasqual G, Chudnovskiy A, Tas JMJ, Agudelo M, Schweitzer LD, Cui A, Hacohen N, Victora GD. Nature. 2018 Jan 25;553(7689):496-500. doi: 10.1038/nature25442. Epub 2018 Jan 17. 10.1038/nature25442 PubMed 29342141