tau exon 9–12 splicing minigene
(Plasmid
#121165)
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PurposeGenerates circular RNAs from a Tau minigene
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 121165 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTau exons 9–12
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Alt nameMAPT
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SpeciesH. sapiens (human)
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Entrez GeneMAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
tau exon 9–12 splicing minigene was a gift from Stefan Stamm (Addgene plasmid # 121165 ; http://n2t.net/addgene:121165 ; RRID:Addgene_121165) -
For your References section:
The human MAPT locus generates circular RNAs. Welden JR, van Doorn J, Nelson PT, Stamm S. Biochim Biophys Acta Mol Basis Dis. 2018 Sep;1864(9 Pt B):2753-2760. doi: 10.1016/j.bbadis.2018.04.023. Epub 2018 May 3. 10.1016/j.bbadis.2018.04.023 PubMed 29729314
Map uploaded by the depositor.
