pcDNA-h-mmPtch1-B-NNQ-HA-GFP
(Plasmid
#121164)
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PurposeExpresses mmPtch1-B NNQ variant
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 121164 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA-h
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePtch1
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SpeciesM. musculus (mouse)
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Mutationdeleted amino acids 620-643, truncated at 1291, NNQ variant
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GenBank IDNM_008957.3
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Entrez GenePtch1 (a.k.a. A230106A15Rik, Ptc, Ptc1, Ptch, mes, wig)
- Promoter CMV
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Tags
/ Fusion Proteins
- HA (C terminal on insert)
- GFP (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-h-mmPtch1-B-NNQ-HA-GFP was a gift from Philip Beachy (Addgene plasmid # 121164 ; http://n2t.net/addgene:121164 ; RRID:Addgene_121164) -
For your References section:
Structural Basis for Cholesterol Transport-like Activity of the Hedgehog Receptor Patched. Zhang Y, Bulkley DP, Xin Y, Roberts KJ, Asarnow DE, Sharma A, Myers BR, Cho W, Cheng Y, Beachy PA. Cell. 2018 Nov 15;175(5):1352-1364.e14. doi: 10.1016/j.cell.2018.10.026. Epub 2018 Nov 8. 10.1016/j.cell.2018.10.026 PubMed 30415841
