pXM03
(Plasmid
#121150)
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PurposeThis plamid was used for biosynthesis of Prenylated 4‑Hydroxybenzoate in E.coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 121150 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET24 b
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameXimB, IDS2
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXM03 was a gift from Minjuan Xu (Addgene plasmid # 121150 ; http://n2t.net/addgene:121150 ; RRID:Addgene_121150) -
For your References section:
Combinatory Biosynthesis of Prenylated 4-Hydroxybenzoate Derivatives by Overexpression of the Substrate-Promiscuous Prenyltransferase XimB in Engineered E. coli. He BB, Bu XL, Zhou T, Li SM, Xu MJ, Xu J. ACS Synth Biol. 2018 Sep 21;7(9):2094-2104. doi: 10.1021/acssynbio.8b00070. Epub 2018 Aug 30. 10.1021/acssynbio.8b00070 PubMed 30103600