pMVA
(Plasmid
#121149)
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PurposeThis plamid was used for improving the production of DMAPP (IPP) in E.coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 121149 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDF- Duet-1
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Streptomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMVA Pathway (ERG12, ERG8, MVD1, IDI, MvaE, MvaS )
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMVA was a gift from Minjuan Xu (Addgene plasmid # 121149 ; http://n2t.net/addgene:121149 ; RRID:Addgene_121149) -
For your References section:
Combinatory Biosynthesis of Prenylated 4-Hydroxybenzoate Derivatives by Overexpression of the Substrate-Promiscuous Prenyltransferase XimB in Engineered E. coli. He BB, Bu XL, Zhou T, Li SM, Xu MJ, Xu J. ACS Synth Biol. 2018 Sep 21;7(9):2094-2104. doi: 10.1021/acssynbio.8b00070. Epub 2018 Aug 30. 10.1021/acssynbio.8b00070 PubMed 30103600
