pET15bpATnp
(Plasmid #121137)

• Purpose: This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.
• Depositing Lab: Keji Zhao
• Publication: Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi: 10.1038/s41467-019-11559-1.

Backbone

• Vector backbone: pET15b
• Total vector size (bp): 8028
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Protein A-Tnp
• Alt name: His6pA-Tnp
• Species: E. coli
• Insert Size (bp): 2388
• Promoter: T7
• Tag / Fusion Protein:
  • his (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7 promoter primer
• 3′ sequencing primer: T7 terminator primer

Resource Information

• Supplemental Documents:
  • pET15bpATnp.gb
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a single bp deletion in LacI that changes the C terminal coding sequence to FPDWKAGSERNAINVS. This change does not affect function.

Please visit https://doi.org/10.1101/571208 for bioRxiv preprint.