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pHTSHP-Smt-IIShhN
(Plasmid #121135)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121135 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHTSHP
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Shh
  • Species
    M. musculus (mouse)
  • Mutation
    Amino acid sequence from 26-195, with two extra isoleucine residues on the N-terminus
  • Entrez Gene
    Shh (a.k.a. 9530036O11Rik, Dsh, HHG-1, Hhg1, Hx, Hxl3, M100081, ShhNC)
  • Promoter tac
  • Tags / Fusion Proteins
    • MBP (N terminal on backbone)
    • HIS (N terminal on backbone)
    • HRV3C site (N terminal on backbone)
    • Smt tag (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHTSHP-Smt-IIShhN was a gift from Philip Beachy (Addgene plasmid # 121135 ; http://n2t.net/addgene:121135 ; RRID:Addgene_121135)

  • For your References section:

    Structural Basis for Cholesterol Transport-like Activity of the Hedgehog Receptor Patched. Zhang Y, Bulkley DP, Xin Y, Roberts KJ, Asarnow DE, Sharma A, Myers BR, Cho W, Cheng Y, Beachy PA. Cell. 2018 Nov 15;175(5):1352-1364.e14. doi: 10.1016/j.cell.2018.10.026. Epub 2018 Nov 8. 10.1016/j.cell.2018.10.026 PubMed 30415841
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