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Tol2-lyzC-mcherry-2a-CDK2-D145N
(Plasmid #121132)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121132 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    tol2 insertion
  • Backbone size w/o insert (bp) 8500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dre-D145N CDK2 DN
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1668
  • Mutation
    D145N point mutation DN
  • Entrez Gene
    cdk2 (a.k.a. wu:fa10c0, wu:fa10c02, zgc:56598, zgc:77684)
  • Promoter neutrophil specific lyzC
  • Tag / Fusion Protein
    • mcherry-2a (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tol2-lyzC-mcherry-2a-CDK2-D145N was a gift from Qing Deng (Addgene plasmid # 121132 ; http://n2t.net/addgene:121132 ; RRID:Addgene_121132)

  • For your References section:

    Phenotypical microRNA screen reveals a noncanonical role of CDK2 in regulating neutrophil migration. Hsu AY, Wang D, Liu S, Lu J, Syahirah R, Bennin DA, Huttenlocher A, Umulis DM, Wan J, Deng Q. Proc Natl Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 10.1073/pnas.1905221116. 10.1073/pnas.1905221116 PubMed 31451657
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