PB-TetON-dual-SunTag-Hygro
(Plasmid
#121119)
-
PurposeEndogenous gene activation with the SunTag CRISPR activation system
-
Depositing Lab
-
Sequence Information
-
Sequences (1) — Accept Affinity Reagent Sequence Policy
-
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 121119 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonePiggyBac
-
Vector typeMammalian Expression, CRISPR
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namedCas9-GCN4x10_scFv-VP64
-
SpeciesM. musculus (mouse), Synthetic
- Promoter Tet-ON
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This PiggyBac vector encodes for the two components of the SunTag system for gene activation. Both transgenes are driven by TetON promoters which also drive resistance to Hygromycin under Doxycycline resistance.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
PB-TetON-dual-SunTag-Hygro was a gift from Pablo Navarro (Addgene plasmid # 121119 ; http://n2t.net/addgene:121119 ; RRID:Addgene_121119) -
For your References section:
The molecular logic of Nanog-induced self-renewal in mouse embryonic stem cells. Heurtier V, Owens N, Gonzalez I, Mueller F, Proux C, Mornico D, Clerc P, Dubois A, Navarro P. Nat Commun. 2019 Mar 7;10(1):1109. doi: 10.1038/s41467-019-09041-z. 10.1038/s41467-019-09041-z PubMed 30846691