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Addgene

Fuw CBX8 3xRA
(Plasmid #121115)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121115 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Fuw
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CBX8 (R19A, R20A, R22A)
  • Species
    H. sapiens (human)
  • Mutation
    R19A, R20A, R22A
  • Entrez Gene
    CBX8 (a.k.a. PC3, RC1)
  • Tag / Fusion Protein
    • V5 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Fuw CBX8 3xRA was a gift from Emily Dykhuizen (Addgene plasmid # 121115 ; http://n2t.net/addgene:121115 ; RRID:Addgene_121115)

  • For your References section:

    Engagement of DNA and H3K27me3 by the CBX8 chromodomain drives chromatin association. Connelly KE, Weaver TM, Alpsoy A, Gu BX, Musselman CA, Dykhuizen EC. Nucleic Acids Res. 2018 Dec 29. pii: 5266947. doi: 10.1093/nar/gky1290. 10.1093/nar/gky1290 PubMed 30597065
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