Fuw CBX8 R19A
(Plasmid
#121112)
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Purposeconditional expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 121112 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFuw
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCBX8 (R19A)
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SpeciesH. sapiens (human)
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MutationR19A
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Entrez GeneCBX8 (a.k.a. PC3, RC1)
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Tag
/ Fusion Protein
- V5 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Fuw CBX8 R19A was a gift from Emily Dykhuizen (Addgene plasmid # 121112 ; http://n2t.net/addgene:121112 ; RRID:Addgene_121112) -
For your References section:
Engagement of DNA and H3K27me3 by the CBX8 chromodomain drives chromatin association. Connelly KE, Weaver TM, Alpsoy A, Gu BX, Musselman CA, Dykhuizen EC. Nucleic Acids Res. 2018 Dec 29. pii: 5266947. doi: 10.1093/nar/gky1290. 10.1093/nar/gky1290 PubMed 30597065