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Addgene

SRF WT pGEX4T
(Plasmid #121100)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121100 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX4T1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SRF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Entrez Gene
    SRF (a.k.a. MCM1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

see attached pdf of map

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SRF WT pGEX4T was a gift from Paul Herring (Addgene plasmid # 121100 ; http://n2t.net/addgene:121100 ; RRID:Addgene_121100)

  • For your References section:

    Identification of Barx2b, a serum response factor-associated homeodomain protein. Herring BP, Kriegel AM, Hoggatt AM. J Biol Chem. 2001 Apr 27;276(17):14482-9. doi: 10.1074/jbc.M011585200. Epub 2001 Jan 29. 10.1074/jbc.M011585200 PubMed 11278942
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