pCUP1-NMGR
(Plasmid #1211)

• Depositing Lab: Susan Lindquist
• Publication: Li et al Science 2000 Jan 28;287(5453):661-4.

Backbone

• Vector backbone: pRS316
• Backbone size w/o insert (bp): 4847
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sup35 NM domain fused with GR
• Alt name: Yeast Sup35
• Alt name: Rat glucocorticoid receptor
• Species: R. norvegicus (rat), S. cerevisiae (budding yeast)
• Insert Size (bp): 3604
• GenBank ID: AY553990
• Entrez Gene: SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)
• Entrez Gene: Nr3c1 (a.k.a. GR, Gcr, Grl)
• Tag / Fusion Protein:
  • GR (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: GR cloned into SacII (5')/SacI site of Lindquist vector 6442 (not destroyed)
• 3′ cloning site: GR in SacII/SacI (3')of Lindquist vector 6442 (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: see comments

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Derived from plasmids from the following reference: Picard D, Khurseed B, Garabedian MJ, Fortin MG, Lindquist S, Yamamoto
KR. Reduced levels of hsp90 compromise steroid receptor action in
vivo. Nature (1990), 348 (6297): 166-8.