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Addgene

ELK1 AB box pcDNA3.1/HisC
(Plasmid #121094)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121094 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1 HisC
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ELK1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    550
  • Entrez Gene
    Elk1 (a.k.a. Elk-1)
  • Tag / Fusion Protein
    • 6xHis-omni-express (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

see attached PDf of map and sequence

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ELK1 AB box pcDNA3.1/HisC was a gift from Paul Herring (Addgene plasmid # 121094 ; http://n2t.net/addgene:121094 ; RRID:Addgene_121094)

  • For your References section:

    Smooth muscle-specific genes are differentially sensitive to inhibition by Elk-1. Zhou J, Hu G, Herring BP. Mol Cell Biol. 2005 Nov . 25(22):9874-85. 10.1128/MCB.25.22.9874-9885.2005 PubMed 16260603
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