ELK1 pcDNA3.1/HisC
(Plasmid
#121093)
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PurposeExpression of full length ELK1 in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 121093 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1 HisC
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameELK1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1290
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Entrez GeneElk1 (a.k.a. Elk-1)
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Tag
/ Fusion Protein
- 6XHis-Omni-xpress (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
see attached PDF of map
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ELK1 pcDNA3.1/HisC was a gift from Paul Herring (Addgene plasmid # 121093 ; http://n2t.net/addgene:121093 ; RRID:Addgene_121093) -
For your References section:
Smooth muscle-specific genes are differentially sensitive to inhibition by Elk-1. Zhou J, Hu G, Herring BP. Mol Cell Biol. 2005 Nov . 25(22):9874-85. 10.1128/MCB.25.22.9874-9885.2005 PubMed 16260603