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Addgene

pUC-fFuCas9-HTBNLS-hph
(Plasmid #121092)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121092 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Vector type
    Bacterial Expression, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9 and hygromycin resist gene
  • Promoter gpdA;trpC

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

a strong constitutive promoter -gpdA promoter to express fFuCas9, which is codon-optimized for efficient expression in Fusarium fujikuroi; a strong constitutive promoter -trpC promoter to express hygromycin resist gene for the selection

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC-fFuCas9-HTBNLS-hph was a gift from Xiao-Jun Ji (Addgene plasmid # 121092 ; http://n2t.net/addgene:121092 ; RRID:Addgene_121092)

  • For your References section:

    CRISPR/Cas9-Based Genome Editing in the Filamentous Fungus Fusarium fujikuroi and Its Application in Strain Engineering for Gibberellic Acid Production. Shi TQ, Gao J, Wang WJ, Wang KF, Xu GQ, Huang H, Ji XJ. ACS Synth Biol. 2019 Feb 15;8(2):445-454. doi: 10.1021/acssynbio.8b00478. Epub 2019 Jan 23. 10.1021/acssynbio.8b00478 PubMed 30616338
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