Skip to main content
Addgene

LV-CIG
(Plasmid #12107)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12107 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    n/a
  • Backbone size w/o insert (bp) 6670
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox
  • Selectable markers
    none

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    nlsCre
  • Alt name
    nls Cre
  • Alt name
    LV-Cre
  • Alt name
    cre
  • Species
    Enterobacteriophage P1
  • Insert Size (bp)
    2476
  • Mutation
    IRES GFP C-terminal to the nlsCre. Bicistronic expression of nlsCre and GFP.
  • Entrez Gene
    cre (a.k.a. P1_gp003)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The cre is from pMC-Cre (Gu et al. 1993 Cell 73, 1155-1164

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LV-CIG was a gift from Inder Verma (Addgene plasmid # 12107)

  • For your References section:

    Delivery of the Cre recombinase by a self-deleting lentiviral vector: efficient gene targeting in vivo. Pfeifer A, Brandon EP, Kootstra N, Gage FH, Verma IM. Proc Natl Acad Sci U S A. 2001 Sep 25. 98(20):11450-5. 10.1073/pnas.201415498 PubMed 11553794
Related items: