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Addgene

Geph CR3m shRNA
(Plasmid #121069)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121069 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mU6pro
  • Vector type
    RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GPHN shRNA
  • gRNA/shRNA sequence
    GAGGAAGUGCAGGCAGUAUGAACUG
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Gphn (a.k.a. Geph)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Insert was originally cloned in Angel de Blas's lab.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

3-point mutations (negative control for shRNA2 targeting gephyrin mRNA, plasmid 121068).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Geph CR3m shRNA was a gift from Angel de Blas (Addgene plasmid # 121069 ; http://n2t.net/addgene:121069 ; RRID:Addgene_121069)

  • For your References section:

    Gephyrin clustering is required for the stability of GABAergic synapses. Yu W, Jiang M, Miralles CP, Li RW, Chen G, de Blas AL. Mol Cell Neurosci. 2007 Dec;36(4):484-500. doi: 10.1016/j.mcn.2007.08.008. Epub 2007 Aug 23. 10.1016/j.mcn.2007.08.008 PubMed 17916433
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