pREVTRE-AID
(Plasmid
#121067)
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Purpose(Empty Backbone) Retrovirus vector expresses AID-fusion protein under TRE promoter and with hygromycin resistance selectio
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 121067 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepREVTRE2
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Backbone manufacturerClontech
- Backbone size (bp) 7256
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Modifications to backboneAID-tag, some restriction enzyme sites removed
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Vector typeMammalian Expression, Retroviral
- Promoter TRE
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Selectable markersHygromycin
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Tag
/ Fusion Protein
- AID (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLNCX5
- 3′ sequencing primer pLNCX3 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pREVTRE-AID was a gift from Randy Poon (Addgene plasmid # 121067 ; http://n2t.net/addgene:121067 ; RRID:Addgene_121067) -
For your References section:
Conditional gene inactivation by combining tetracycline-mediated transcriptional repression and auxin-inducible degron-mediated degradation. Ng LY, Ma HT, Liu JCY, Huang X, Lee N, Poon RYC. Cell Cycle. 2018 Dec 23. doi: 10.1080/15384101.2018.1563395. 10.1080/15384101.2018.1563395 PubMed 30582405
