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Addgene

LV-Cre
(Plasmid #12106)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12106 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    p156RRL
  • Backbone manufacturer
    Cell Genesys
  • Backbone size w/o insert (bp) 7424
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    nlsCre
  • Alt name
    nls Cre
  • Alt name
    LV-Cre
  • Alt name
    cre
  • Species
    Enterobacteriophage P1
  • Insert Size (bp)
    1136
  • Entrez Gene
    cre (a.k.a. P1_gp003)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer LNCX
    • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    The cre is from pMC-Cre (Gu et al. 1993 Cell 73, 1155-1164
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The nlsCre was first cloned into the EcoRV site of a pBluescript II KS vector, and then inserted into the XbaI-SalI sites of a pRRL-derived lentiviral vector that contains a central polypurine tract (ppt) and the posttranscriptional element of the woodchuck hepatitis virus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LV-Cre was a gift from Inder Verma (Addgene plasmid # 12106)

  • For your References section:

    Delivery of the Cre recombinase by a self-deleting lentiviral vector: efficient gene targeting in vivo. Pfeifer A, Brandon EP, Kootstra N, Gage FH, Verma IM. Proc Natl Acad Sci U S A. 2001 Sep 25. 98(20):11450-5. 10.1073/pnas.201415498 PubMed 11553794
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