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GFP-BioID-FAM21
(Plasmid #121046)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121046 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcGFP1-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 9687
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    BioID
  • Alt name
    BirA*
  • Species
    E. Coli
  • Insert Size (bp)
    1116
  • Mutation
    R118G (highly promiscuous form)
  • GenBank ID
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site Kpn1 (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    FAM21C
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3963
  • GenBank ID
    NM_004637.5
  • Entrez Gene
    FAM21C
  • Promoter CMV

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site kpn1 (not destroyed)
  • 3′ cloning site bamh1 (not destroyed)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-BioID-FAM21 was a gift from Gia Voeltz (Addgene plasmid # 121046 ; http://n2t.net/addgene:121046 ; RRID:Addgene_121046)

  • For your References section:

    A Novel Class of ER Membrane Proteins Regulates ER-Associated Endosome Fission. Hoyer MJ, Chitwood PJ, Ebmeier CC, Striepen JF, Qi RZ, Old WM, Voeltz GK. Cell. 2018 Sep 20;175(1):254-265.e14. doi: 10.1016/j.cell.2018.08.030. Epub 2018 Sep 13. 10.1016/j.cell.2018.08.030 PubMed 30220460
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