3XFlag-TMCC1
(Plasmid #121045)

• Purpose: Expresses human TMCC1 in mammalian cells
• Depositing Lab: Gia Voeltz
• Publication: Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. doi: 10.1016/j.cell.2018.08.030. Epub 2018 Sep 13.

Backbone

• Vector backbone: pAcGFP1-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5993
• Modifications to backbone: 3xFlag tag was amplified and inserted into the NheI and XhoI sites in order to replace the GFP.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Trans membrane and coiled coil domain containing protein 1
• Alt name: TMCC1
• Alt name: KIAA0779
• Species: H. sapiens (human)
• Insert Size (bp): 1962
• GenBank ID: NM_001349265.1
• Entrez Gene: TMCC1
• Promoter: CMV
• Tag / Fusion Protein:
  • 3x-Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (not destroyed)
• 3′ cloning site: Kpn1 (not destroyed)
• 5′ sequencing primer: NA
• 3′ sequencing primer: TCT AGG GGA TGA AAA GAA CCG TTC CAC ATA GC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry