pCB6-HFE-EGFP
(Plasmid #12104)

• Depositing Lab: Pamela Bjorkman
• Publication: Giannetti et al J Biol Chem. 2004 Jun 11. 279(24):25866-75.

Backbone

• Vector backbone: pCB6
• Backbone manufacturer: Ira Mellman, Yale University
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HFE
• Alt name: hemochromatosis protein
• Alt name: MHC class I-like protein HFE
• Alt name: hereditary hemochromatosis protein HLA-H
• Species: H. sapiens (human)
• Insert Size (bp): 1044
• GenBank ID: Q30201
• Entrez Gene: HFE (a.k.a. HFE1, HH, HLA-H, MVCD7, TFQTL2)
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry