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pET23a-LIR-1 D1-D2
(Plasmid #12103)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12103 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET23a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3666
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Leukocyte immunoglobulin-like receptor 1
  • Alt name
    LIR-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    600
  • Mutation
    Amino acids 1−198*
  • GenBank ID
    AF009220
  • Entrez Gene
    LILRB1 (a.k.a. CD85J, ILT-2, ILT2, LIR-1, LIR1, MIR-7, MIR7, PIR-B, PIRB)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*Note that these amino acid assignments are based on crystal structure. See the following sequence ID in NCBI for reference: pdb|1UGN|A

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET23a-LIR-1 D1-D2 was a gift from Pamela Bjorkman (Addgene plasmid # 12103 ; http://n2t.net/addgene:12103 ; RRID:Addgene_12103)

  • For your References section:

    Crystal structure of HLA-A2 bound to LIR-1, a host and viral major histocompatibility complex receptor. Willcox BE, Thomas LM, Bjorkman PJ. Nat Immunol. 2003 Sep . 4(9):913-9. 10.1038/ni961 PubMed 12897781
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