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Addgene

CMV AP-4
(Plasmid #12101)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12101 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    modified pBluescript SK+
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AP-4
  • Alt name
    AP4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1270
  • Entrez Gene
    TFAP4 (a.k.a. AP-4, bHLHc41)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PvuI (destroyed during cloning)
  • 3′ cloning site BglI (destroyed during cloning)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Diagnostic digest with XhoI and SphI should release a 1.3kb band. See author's map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV AP-4 was a gift from Robert Tjian (Addgene plasmid # 12101 ; http://n2t.net/addgene:12101 ; RRID:Addgene_12101)

  • For your References section:

    Transcription factor AP-4 contains multiple dimerization domains that regulate dimer specificity. Hu YF, Luscher B, Admon A, Mermod N, Tjian R. Genes Dev. 1990 Oct . 4(10):1741-52. 10.1101/gad.4.10.1741 PubMed 2123466
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