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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 12100 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneSP72
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3500
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAP-2
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Alt nameAP2
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SpeciesH. sapiens (human)
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MutationL2V
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Entrez GeneTFAP2A (a.k.a. AP-2, AP-2alpha, AP2TF, BOFS, TFAP2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site see map (unknown if destroyed)
- 3′ cloning site see map (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SP (RSV) AP-2 was a gift from Robert Tjian (Addgene plasmid # 12100 ; http://n2t.net/addgene:12100 ; RRID:Addgene_12100) -
For your References section:
Analysis of the DNA-binding and activation properties of the human transcription factor AP-2. Williams T, Tjian R. Genes Dev. 1991 Apr . 5(4):670-82. 10.1101/gad.5.4.670 PubMed 2010091