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Addgene

pBS Sp1 full length
(Plasmid #12096)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12096 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sp1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4400
  • Entrez Gene
    SP1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13pUC-Fwd [5'-CCCAGTCACGACGTTGTAAAACG-3']
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map for more information.

This particular clone corresponds to the first 4237 nucleotides of GenBank reference sequence NM_138473.2 and contains the entire coding sequence of Sp1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS Sp1 full length was a gift from Robert Tjian (Addgene plasmid # 12096 ; http://n2t.net/addgene:12096 ; RRID:Addgene_12096)

  • For your References section:

    Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain. Kadonaga JT, Carner KR, Masiarz FR, Tjian R. Cell. 1987 Dec 24. 51(6):1079-90. 10.1016/0092-8674(87)90594-0 PubMed 3319186
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