His6x-EX-HA-FRB-MBD_pYFJ16
(Plasmid
#120917)
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PurposeExpresses His6-EX-HA-FRB-MBD in bacteria for protein purification
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 120917 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYFJ16
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHis6-EX-HA-FRB-MBD
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
His6x-EX-HA-FRB-MBD_pYFJ16 was a gift from Alice Ting (Addgene plasmid # 120917 ; http://n2t.net/addgene:120917 ; RRID:Addgene_120917) -
For your References section:
Directed Evolution of Split APEX2 Peroxidase. Han Y, Branon TC, Martell JD, Boassa D, Shechner D, Ellisman MH, Ting A. ACS Chem Biol. 2019 Mar 8. doi: 10.1021/acschembio.8b00919. 10.1021/acschembio.8b00919 PubMed 30848125