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Addgene

pcDNA-h-mmPtch1-B-L235C-HA
(Plasmid #120906)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120906 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA-h
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ptch1
  • Species
    M. musculus (mouse)
  • Mutation
    deleted amino acids 620-643, truncated at 1291, L235C
  • GenBank ID
    NM_008957.3
  • Entrez Gene
    Ptch1 (a.k.a. A230106A15Rik, Ptc, Ptc1, Ptch, mes, wig)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-h-mmPtch1-B-L235C-HA was a gift from Philip Beachy (Addgene plasmid # 120906 ; http://n2t.net/addgene:120906 ; RRID:Addgene_120906)

  • For your References section:

    Structural Basis for Cholesterol Transport-like Activity of the Hedgehog Receptor Patched. Zhang Y, Bulkley DP, Xin Y, Roberts KJ, Asarnow DE, Sharma A, Myers BR, Cho W, Cheng Y, Beachy PA. Cell. 2018 Nov 15;175(5):1352-1364.e14. doi: 10.1016/j.cell.2018.10.026. Epub 2018 Nov 8. 10.1016/j.cell.2018.10.026 PubMed 30415841
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