pHH29_32 series plasmid
(Plasmid #120901)

• Purpose: Also known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated device
• Depositing Lab: Domitilla Del Vecchio
• Publication: Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi: 10.1038/s41467-018-07899-z.

Backbone

• Vector backbone: p15A plasmid
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Ec-TTL-P109
• Insert Size (bp): 45

Gene/Insert 2

• Gene/Insert name: sRNA-A targeting sequence
• Insert Size (bp): 15

Gene/Insert 3

• Gene/Insert name: RBS TIR=974 of GFPop1 gene
• Insert Size (bp): 23

Gene/Insert 4

• Gene/Insert name: GFPop1
• Insert Size (bp): 720

Gene/Insert 5

• Gene/Insert name: RBS TIR=6474 of ECF32mut gene
• Insert Size (bp): 26

Gene/Insert 6

• Gene/Insert name: ECF32mut
• Insert Size (bp): 549

Gene/Insert 7

• Gene/Insert name: T7 terminator
• Insert Size (bp): 48

Gene/Insert 8

• Gene/Insert name: resource competitor

Resource Information

• Supplemental Documents:
  • pHH29_32_HG27.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry