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Addgene

pHH04_32_sRNA-A_Sp_coD
(Plasmid #120900)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120900 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    ColE2 plasmid
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    pECF32 promoter [i.e., Pecf32_1122 (-60 to -1)]
  • Insert Size (bp)
    60

Gene/Insert 2

  • Gene/Insert name
    sRNA-A
  • Insert Size (bp)
    113

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHH04_32_sRNA-A_Sp_coD was a gift from Domitilla Del Vecchio (Addgene plasmid # 120900 ; http://n2t.net/addgene:120900 ; RRID:Addgene_120900)
  • For your References section:

    A quasi-integral controller for adaptation of genetic modules to variable ribosome demand. Huang HH, Qian Y, Del Vecchio D. Nat Commun. 2018 Dec 21;9(1):5415. doi: 10.1038/s41467-018-07899-z. 10.1038/s41467-018-07899-z PubMed 30575748