pSico p53
(Plasmid #12089)

• Depositing Lab: Tyler Jacks
• Publication: Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5.

Backbone

• Vector backbone: pSico
• Backbone size w/o insert (bp): 7560
• Vector type: Mammalian Expression, Lentiviral, RNAi, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p53 shRNA
• Alt name: p53
• Species: M. musculus (mouse)
• Insert Size (bp): 55
• Mutation: EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. Cre causes EGFP to be recombined out of the construct, activating p53 shRNA expression.
• Entrez Gene: Trp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (destroyed during cloning)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: EGFP-C

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cre TURNS ON p53 shRNA expression. The shRNA is directed against the mouse p53 sequence 5'-GTACTCTCCTCCCCTCAAT-3'.

Follow safety guidelines when handling live virus expressing p53 shRNA.