ER-moxMaple3
(Plasmid #120885)

• Purpose: mammalian expression of ER localized moxMaple3
• Depositing Lab: Erik Snapp
• Publication: Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.1038/s41598-018-32955-5.

Backbone

• Vector backbone: ER-mRFP
• Backbone manufacturer: Addgene plasmid 62236
• Modifications to backbone: ER-RFP contains fusions of the bovine prolactin signal sequence, mRFP, and a KDEL ER retention sequence. mRFP was replaced with moxMaple3
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: moxMaple3
• Species: Synthetic
• Promoter: CMV
• Tags / Fusion Proteins:
  • bovine prolactin signal sequence (N terminal on backbone) (N terminal on backbone)
  • KDEL ER retention sequence (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please Note: Plasmid contains a 72 basepair deletion in the SV40 promoter sequence upstream of NeoR/KanR compared to the ER-mRFP vector backbone. This deletion is not known to affect plasmid function.