pmCherry-T2A-moxMaple3
(Plasmid
#120875)
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PurposeMammalian expression of mCherry and moxMaple3
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 120875 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmCherry
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Backbone manufacturerderived from Clontech N1-EGFP (clontech)
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namemCherry
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SpeciesSynthetic
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Mutationfrom mMaple3, moxMaple3 was engineered by replacing Cysteines 110 and 180 with Alanines or Valines, and the consensus N-linked Asparagines with Glutamine and Aspartic acid
Gene/Insert 2
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Gene/Insert namemoxMaple3
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SpeciesSynthetic
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmCherry-T2A-moxMaple3 was a gift from Vladislav Verkhusha (Addgene plasmid # 120875 ; http://n2t.net/addgene:120875 ; RRID:Addgene_120875) -
For your References section:
moxMaple3: a Photoswitchable Fluorescent Protein for PALM and Protein Highlighting in Oxidizing Cellular Environments. Kaberniuk AA, Mohr MA, Verkhusha VV, Snapp EL. Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.1038/s41598-018-32955-5. 10.1038/s41598-018-32955-5 PubMed 30283009