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Addgene

pmCherry-T2A-moxMaple3
(Plasmid #120875)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120875 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pmCherry
  • Backbone manufacturer
    derived from Clontech N1-EGFP (clontech)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    mCherry
  • Species
    Synthetic
  • Mutation
    from mMaple3, moxMaple3 was engineered by replacing Cysteines 110 and 180 with Alanines or Valines, and the consensus N-linked Asparagines with Glutamine and Aspartic acid

Gene/Insert 2

  • Gene/Insert name
    moxMaple3
  • Species
    Synthetic

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCherry-T2A-moxMaple3 was a gift from Vladislav Verkhusha (Addgene plasmid # 120875 ; http://n2t.net/addgene:120875 ; RRID:Addgene_120875)
  • For your References section:

    moxMaple3: a Photoswitchable Fluorescent Protein for PALM and Protein Highlighting in Oxidizing Cellular Environments. Kaberniuk AA, Mohr MA, Verkhusha VV, Snapp EL. Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.1038/s41598-018-32955-5. 10.1038/s41598-018-32955-5 PubMed 30283009